Work of Chromatography – Basic Principle of Chromatography

Chromatography is a technique which is used to separate component of the mixture. Chromatography derives from a Greek word. Chrome mean Color and Graphy mean writing. Khromatos mean color writing. First Mikhail Tsvet worked on plant pigments like Chlorophyll, Xanthophyll, and carotenoids in 1900.  He separated plants Pigments. Many types of Chromatography techniques are used …

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Nicotine Effects on Brain

Nicotine Effects on Brain are very worst. Persons who called chain smoker are addict of smoking. Because it act as Stimulant because excessive use of smoking leads to increase Nicotine receptors which release Dopamine. One who took cigarette he fell pleasure and start to used smoking. Brain Brain is the vital part of human nervous …

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Liver Cancer- Hepatocellular Carcinoma – BioMadam

Hepatitis Human beings are unprotected to the risk of several diseases. A large number of   our population is being target by many diseases. Death rates are increase rapidly that is associated with dangerous diseases. Human beings are suffering from many diseases. To reduce the diseases and death rates efforts are continued. Due to the knowledge …

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Types of PCR – Common Kinds of Polymerase Chain Reaction

PCR is the common type of scientific tool used for amplification of Genomic DNA. Different types of PCR used in labs due to their specificity and sensitivity. This PCR used for the qualitative and quantitative test. PCR Steps are involved de-maturation, annealing, and Extension.  Primers, Taq Polymerase, and nucleotides are used. List of Different types …

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Cancer Biomarkers – micerRNAs – miRNA 21 – miRNA 122

MicroRNA is defined as noncoding small RNAs plying major role in gene regulation at post transcription level. Mature microRNA contains 22 nucleotide and shot in length. miRNAs shows expression in cancer diagnosis. Its Biogenesis involved as, Pri-microRNA is transcribed by RNA polymerase 11 after that precursor microRNA is formed by Drosha and dicer is involved …

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DNA Sequencing Method [Updated with Maxam Gilbert Method]

The base sequence of DNA fragments that have been cloned can be determined  In these methods nucleotide sequence of DNA is to be determined. DNA to Protein Sequence can be find out by Expasy Tool. Basic Principle To check DNA sequencing appropriate methods are used to generate DNA fragments that end at the four bases …

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