Work of Chromatography – Basic Principle of Chromatography

Chromatography is a technique which is used to separate component of the mixture. Chromatography derives from a Greek word. Chrome mean Color and Graphy mean writing. Khromatos mean color writing. First Mikhail Tsvet worked on plant pigments like Chlorophyll, Xanthophyll, and carotenoids in 1900.  He separated plants Pigments. Many types of Chromatography techniques are used …

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Nicotine Effects on Brain

Nicotine Effects on Brain are very worst. Persons who called chain smoker are addict of smoking. Because it act as Stimulant because excessive use of smoking leads to increase Nicotine receptors which release Dopamine. One who took cigarette he fell pleasure and start to used smoking. Brain Brain is the vital part of human nervous …

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Types of PCR – Common Kinds of Polymerase Chain Reaction

PCR is the common type of scientific tool used for amplification of Genomic DNA. Different types of PCR used in labs due to their specificity and sensitivity. This PCR used for the qualitative and quantitative test. PCR Steps are involved de-maturation, annealing, and Extension.  Primers, Taq Polymerase, and nucleotides are used. List of Different types …

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DNA Sequencing Method [Updated with Maxam Gilbert Method]

The base sequence of DNA fragments that have been cloned can be determined  In these methods nucleotide sequence of DNA is to be determined. DNA to Protein Sequence can be find out by Expasy Tool. Basic Principle To check DNA sequencing appropriate methods are used to generate DNA fragments that end at the four bases …

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Polymerase Chain Reaction – PCR Steps

Polymerase chain reaction is involved replication of DNA. This is fast and reliable method in which minute copies of genetic material can be amplified millions of times. Primers and Taq polymerase are used for this purpose and Gel electropherosis helps to visualized DNA product. PCR is a very sensitive technique to be used. This is …

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Plant DNA Extraction – CTAB DNA Extraction Protocol

Deoxyribonucleic acid  is thread like structure made up of nucleotide having genetic information. This is found in every organism. DNA is a heredity material. The information is stored in the form of codes. These codes made up of four type of bases. The important thing is that DNA can replicate and can make copies. And …

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