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Chemistry

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The technique of column chromatography can be defined in two ways in accordance with the field of science in which it is being utilized – Chemistry and Biology. When applied in chemistry, it is used to separate one or sometimes more chemical compounds from a mixture dissolved in an appropriate solvent. Whereas in biology, it most often is employed to separate a biological compound such as nucleic acids or Proteins from a mixture (also dissolved…

Thin-layer chromatography (TLC) is a separation technique for a mixture of non-volatile compounds monitored by their rate of movement on a thin layer of adsorbent coated a plate or sheet. There are different types of sheets used in TLC, of which most common are plastic, glass, and aluminum foil while the most common types of coatings (absorbent materials) used are silica gel, cellulose, and aluminum oxide. The adsorbent layer is termed as a stationary phase…

A chemist is a scientist who studies and does research in chemistry and its various processes. In broader terms, chemists study matter, its properties, their composition, the chemical reactions happening and how those chemical reactions result in new products and so on. To celebrate the necessity of chemistry and its sub-fields and how this field has revolutionized and transformed during the last decades, the top scientist of the field are being presented here along with…

Organic compounds constitute a large class of chemical compounds characterized by the presence of carbon atoms as essential and main components of them. The exceptions include carbides, cyanides, and carbonates. Organic compounds are copiously used in our daily life, ranging from the food and drinks we take to the air we breathe. Let us now look closely at how organic compounds are being utilized in their various forms and have become an essential to our…

In its most archetypical definition, compounds having carbon atom as their central element or backbone in association with various other non-metal elements are known as organic compounds. The carbon atoms are linked with other atoms through covalent bonds and most common elements forming associations with carbon backbone include hydrogen, oxygen, and nitrogen. There are few exceptions of carbon-containing compounds which are not classified in the category of organic compounds. These examples of these include cyanides…

Thin layer chromatography (TLC) is a chromatographic separation technique used to separate non-volatile compounds from a mixture. It utilizes a thin layer of a material on which an adsorbent is coated (stationary phase) to attach the compound being separated or analyzed in the liquid mixture (mobile phase). While there are many advantages associated with this technique, it also offers some disadvantages or limitations which must be kept in mind when one plans to include this…