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Molecular Biology

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Thin-layer chromatography (TLC) is a separation technique for a mixture of non-volatile compounds monitored by their rate of movement on a thin layer of adsorbent coated a plate or sheet. There are different types of sheets used in TLC, of which most common are plastic, glass, and aluminum foil while the most common types of coatings (absorbent materials) used are silica gel, cellulose, and aluminum oxide. The adsorbent layer is termed as a stationary phase…

Thin layer chromatography (TLC) is a chromatographic separation technique used to separate non-volatile compounds from a mixture. It utilizes a thin layer of a material on which an adsorbent is coated (stationary phase) to attach the compound being separated or analyzed in the liquid mixture (mobile phase). While there are many advantages associated with this technique, it also offers some disadvantages or limitations which must be kept in mind when one plans to include this…

Ion exchange chromatography alias ion chromatography is an analytical technique used frequently for the separation and identification of the ionic compounds. It is a process of the separation of ionic and polar compounds based on their interaction with another ionic substance typically known as ion exchanger. Not only for ionic solutions, but it also can be used for the separation of any type of charged molecule e.g., amino acids, nucleic acids and proteins. There are…

The technique of the separation of a mixture of compounds into its individual components is known as chromatography. When talking about the biochemical mixtures or compounds, a modified chromatographic technique is used known as affinity chromatography. In the most basic definition, affinity chromatography depends on the interaction of one biomolecule with another (hence the name affinity) and hence utilized this interaction to carry out the separation of the molecules. The types of molecular interactions on…

Chromatography is a technique which is used to separate component of the mixture. Chromatography derives from a Greek word. Chrome mean Color and Graphy mean writing. Khromatos mean color writing. First Mikhail Tsvet worked on plant pigments like Chlorophyll, Xanthophyll, and carotenoids in 1900. He separated plants Pigments. Many types of Chromatography techniques are used named are. Biochemical techniques may be Preparative or Analytical. Preparative Procedure is most commonly used to a purification of a…

Hepatitis Human beings are unprotected to the risk of several diseases. A large number of   our population is being target by many diseases. Death rates are increase rapidly that is associated with dangerous diseases. Human beings are suffering from many diseases. To reduce the diseases and death rates efforts are continued. Due to the knowledge of causality of deaths and diseases the awareness of peoples are being continue regarding these factors which causing these diseases…