Work of Chromatography – Basic Principle of Chromatography

Chromatography is a technique which is used to separate component of the mixture. Chromatography derives from a Greek word. Chrome mean Color and Graphy mean writing. Khromatos mean color writing. First Mikhail Tsvet worked on plant pigments like Chlorophyll, Xanthophyll, and carotenoids in 1900.  He separated plants Pigments. Many types of Chromatography techniques are used …

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Nicotine Effects on Brain

Nicotine Effects on Brain are very worst. Persons who called chain smoker are addict of smoking. Because it act as Stimulant because excessive use of smoking leads to increase Nicotine receptors which release Dopamine. One who took cigarette he fell pleasure and start to used smoking. Brain Brain is the vital part of human nervous …

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Liver Cancer- Hepatocellular Carcinoma – BioMadam

Hepatitis Human beings are unprotected to the risk of several diseases. A large number of   our population is being target by many diseases. Death rates are increase rapidly that is associated with dangerous diseases. Human beings are suffering from many diseases. To reduce the diseases and death rates efforts are continued. Due to the knowledge …

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Types of PCR – Common Kinds of Polymerase Chain Reaction

PCR is the common type of scientific tool used for amplification of Genomic DNA. Different types of PCR used in labs due to their specificity and sensitivity. This PCR used for the qualitative and quantitative test. PCR Steps are involved de-maturation, annealing, and Extension.  Primers, Taq Polymerase, and nucleotides are used. List of Different types …

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Cancer Biomarkers – micerRNAs – miRNA 21 – miRNA 122

MicroRNA is defined as noncoding small RNAs plying major role in gene regulation at post transcription level. Mature microRNA contains 22 nucleotide and shot in length. miRNAs shows expression in cancer diagnosis. Its Biogenesis involved as, Pri-microRNA is transcribed by RNA polymerase 11 after that precursor microRNA is formed by Drosha and dicer is involved …

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Branches of Biology – Importance of Biology

What is Biology? Biology is the branch of science and also known as science of life. The word Biology is comprised of two Greek words “bios” (life) and “logos” (study), and is defined as the science of life and living organisms. Biological science gives understanding on vast scale, ranging from the molecular mechanisms in cells, …

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Facts about Mitochondria

Mitochondria is the powerhouse of the cell. It is an organelle like other organelles including endoplasmic reticulum, ribosomes and nucleus. It is found in the cell of eukaryotic organisms. Definition It is an organelle that are found in most of the eukaryotic cell having cellular processes. It has double membrane,  outer membrane  and inner membrane …

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DNA Sequencing Method [Updated with Maxam Gilbert Method]

The base sequence of DNA fragments that have been cloned can be determined  In these methods nucleotide sequence of DNA is to be determined. DNA to Protein Sequence can be find out by Expasy Tool. Basic Principle To check DNA sequencing appropriate methods are used to generate DNA fragments that end at the four bases …

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What are Proteins? [Updated] Structure and classification of Proteins

Technically these are macromolecules are formed when amino acid unit link together. These are very essential for living organism. Proteins are involved in many chemical processes and have great nutritional value. In 1838 Jons Jacob introduced the term. The word, “Proteins” derived from Greek word “Proteios”. These are specific specie to specie. These are species …

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Classifications of Enzymes – Characteristics and Structure of Enzymes

What are Enzymes? Enzymes are very important group of bio molecules synthesized by living cells. They act as a catalyst. Enzymes accelerate the rate of reaction. Classified in to 6 groups. What does enzyme do? Enzyme increases the rate of reaction It does not participate in reaction it just accelerate reaction Where enzymes are made? Enzymes …

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