A typical living cell is specified for regular functioning in a body. Similarly, there are genes in DNA that encrypt protein molecules. It’s quite a complex procedure when a gene converts to particular proteins to perform functions afterwards. It consists of two significant steps, such as:
- Transcription
- Translation
There are different translation tools available that help a DNA translate its coding directly to proteins. One such tool is an EXPASY Translate Tool which permits a nucleotide to create a protein sequence.
We have provided different protein sequencing using this tool below, so let’s check them out!
Results of Expasy Translation
Sus scrofa gap junction protein alpha 1 (GJA1), mRNA
>NM_001244212.1 Sus scrofa gap junction protein alpha 1 (GJA1), mRNA
Translate Tool – Results of translation
Open reading frames are highlighted in red. Please select one of the following frames – in the next page, you will be able to select your initiator and retrieve your amino acid sequence:
5’3′ Frame 1
T R Y Q H F S F I R G K E Stop G K Y Q T A A D F Stop T L N R Q V Stop V P E L A F S F Y F I L Stop G V
5’3′ Frame 2
R G I S T F L S L G G R S E E S T K Q Q Q T F K L Stop I D R S E C L N L L F H F T S S S E A C
5’3′ Frame 3
E V S A L F F H Stop G E G V R K V P N S S R L L N F K Stop T G L S A Stop T C F F I L L H P L R R A
3’5′ Frame 1
G T P Q R Met K Stop N E K A S S G T Q T C L F K V Stop K S A A V W Y F P H S F P L Met K E K C Stop Y L
3’5′ Frame 2
A R L R G Stop S K Met K K Q V Q A L R P V Y L K F K S L L L F G T F L T P S P Stop Stop K K S A D T S
3’5′ Frame 3
H A S E D E V K Stop K S K F R H S D L S I Stop S L K V C C C L V L S S L L P P N E R K V L I P
DNA to Protein Translation
Protein Sequence
Frame 1
I L L L G T A V E S A W G D E Q S A F R C N T Q Q P G C E N V C Y D K S F P I S H V R F W V L Q I I 50
F V S V P T L L Y L A H V F Y V M R K E * S R * K * R S L S T A L R S T A R * K C E G
Total length= 93
Map
1 A U C C U G C U C U U G G G C A C A G C U G U U G A G U C A G C C U G G G G U G A U G A G C A G U C U G C C U U U C G U 60
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U A G G A C G A G A A C C C G U G U C G A C A A C U C A G U C G G A C C C C A C U A C U C G U C A G A C G G A A A G C A
I L L L G T A V E S A W G D E Q S A F R
S C S W A Q L L S Q P G V M S S L P F V
P A L G H S C * V S L G * * A V C L S L
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D Q E Q A C S N L * G P T I L L R G K T
G A R P C L Q Q T L R P H H A T Q R E N
R S K P V A T S D A Q P S S C D A K R Q 61 U G U A A C A C U C A G C A A C C C G G U U G U G A A A A U G U C U G C U A U G A C A A A U C C U U C C C C A U C U C U 120
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A C A U U G U G A G U C G U U G G G C C A A C A C U U U U A C A G A C G A U A C U G U U U A G G A A G G G G U A G A G A
C N T Q Q P G C E N V C Y D K S F P I S
V T L S N P V V K M S A M T N P S P S L
* H S A T R L * K C L L * Q I L P H L S
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T V S L L G T T F I D A I V F G E G D R
Y C E A V R N H F H R S H C I R G W R E
L V * C G P Q S F T Q * S L D K G M E * 121 C A C G U G C G U U U C U G G G U C C U G C A G A U C A U A U U U G U G U C U G U U C C U A C A C U C U U G U A C C U G 180
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G U G C A C G C A A A G A C C C A G G A C G U C U A G U A U A A A C A C A G A C A A G G A U G U G A G A A C A U G G A C
H V R F W V L Q I I F V S V P T L L Y L
T C V S G S C R S Y L C L F L H S C T W
R A F L G P A D H I C V C S Y T L V P G
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V H T E P D Q L D Y K H R N R C E Q V Q
R A N R P G A S * I Q T Q E * V R T G P
T R K Q T R C I M N T D T G V S K Y R A 181 G C U C A U G U G U U C U A C G U G A U G C G A A A G G A A U A A A G C A G A U A G A A A U A A A G A A G U U U A A G U 240
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C G A G U A C A C A A G A U G C A C U A C G C U U U C C U U A U U U C G U C U A U C U U U A U U U C U U C A A A U U C A
A H V F Y V M R K E * S R * K * R S L S
L M C S T * C E R N K A D R N K E V * V
S C V L R D A K G I K Q I E I K K F K Y
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S M H E V H H S L F L A S L F L S T * T
E H T R R S A F P I F C I S I F F N L Y
* T N * T I R F S Y L L Y F Y L L K L V 241 A C G G C A U U G A G G A G C A C G G C A A G G U G A A A A U G C G A G G G G G 280
—————————————-
U G C C G U A A C U C C U C G U G C C G U U C C A C U U U U A C G C U C C C C C
T A L R S T A R * K C E G
R H * G A R Q G E N A R G
G I E E H G K V K M R G
—————————————-
R C Q P A R C P S F A L P
P M S S C P L T F I R P P
A N L L V A L H F
MBOSS TRANSEQ
Sus scrofa gap junction protein alpha 1 (GJA1), mRNA
>NM_001244212.1 Sus scrofa gap junction protein alpha 1 (GJA1), mRNA
A C G A G G T A T C A G C A C T T T T C T T T C A T T A G G G G G A A G G A G T G A G G A A A G T A C C A A A C A G C A G C A G A C T T T T
A A A C T T T A A A T A G A C A G G T C T G A G T G C C T G A A C T T G C T T T T T C A T T T T A C T T C A T C C T C T G A G G C G T G C C
T A C T T C A C T T C T T T T A A G G G A A A G A G T A G T G T C C G G G C A A C A T G G G T G A C T G G A G T G C C T T A G G C A A A C T
C C T T G A C A A G G T T C A A G C C T A T T C C A C C G C T GG A G G G A A G G T G T G G C T G T C A G T C C T T T T C A T T T T C C G A
A T C C T G C T C T T G G G C A C A G C T G T T G A G T C A G C C T G G G G T G A T G A G C A G T C T G C C T T T C G T T G T A A C A C T C
A G C A A C C C G G T T G T G A A A A T G T C T G C T A T G A C A A A T C C T T C C C C A T C T C T C A C G T G C G T T T C T G G G T C C T
G C A G A T C A T A T T T G T G T C T G T T C C T A C A C T C T T G T A C C T G G C T C A T G T G T T C T A C G T G A T G C G A A A G G A A
G A G A A A C T G A A C A A G A A A G A G G A G G A A C T C A A A G T T G C C C A A A C T G A C G G T G T C A A C G T G G A G A T G C A C T
T A A A G C A G A T A G A A A T A A A G A A G T T T A A G T A C G G C A T T G A G G A G C A C G G C A A G G T G A A A A T G C G A G G G G G
C T T G C T G C G A A C C T A C A T C A T C A G T A T C C T C T T C A A G T C T G T C T T C G A G G T G G C C T T C T T G C T G A T C C A A
T G G T A C A T C T A T G G A T T C A G C C T G A G T G C T G T T T A C A C T T G C A A A A G A G A T C C C T G C C C G C A C C A G G T G G
A C T G T T T C C T C T C T C G T C C C A C G G A G A A A A C C A T C T T C A T C A T C T T C A T G C T G G T C G T A T C C T T G G T G T C
T C T C G C C T T G A A C A T C A T C G A A C T C T T C T A T G T C T T C T T C A A G G G T G T T A A G G A T C G T G T G A A G G G A A A G
A G T G A T C C T T A C C A C G C C A C C A C C G G T C C A C T G A G C C C C T C C A A A G A C T G T G G A T C T C C A A A A T A C G C T T
A T T T C A A T G G C T G C T C C T C A C C A A C T G C T C C G C T C T C A C C C A T G T C C C C T C C C G G A T A C A A G C T G G T T A C
C G G A G A C A G A A A C A A T T C T T C C T G C C G C A A T T A C A A C A A A C A A G C G A G T G A G C A A A A C T G G G C T A A T T A C
A G T G C C G A G C A G A A T C G A A T G G G G C A G G C A G G A A G C A C C A T C T C T A A C T C C C A T G C AC A G C C T T T T G A T T
T C C C C G A T G A C A A C C A G A A T T C T A A A A A A C T C G A T G C T G G C C A T G A A C T C C A G C C T C T A G C C A T T G T G G A
C C A G C G G C C T T C C A G C A G A G C C A G C A G T C G C G C C A G C A G C C G A C C T C G G C C T G A T G A C C T G G A G A T C T A G
A G C C A A T C T T G A G A A C A T C A A G A T T C C A C T C A G T T G T G G A G A A G A A A A A G G T G C T A T A G A A A G T G C A C C T
T A G G T G T T C A T T T T T T T T C C A G T G G A G G T G G T A C T C A A C A G C C T T G T C A C A A G G C T T A G A A A A C A C A A A G
A C A T T A G A A T A C C T G G T T C A T A G G G G G T G T T A G G G A T A G G T G G G T G G A G A G G G A G G G G A T G A G G G A G G T A
C A G G T T G G T A T T T A A T G T A G T T G A C T T A A A G A A C T T A A G T T C T A A A T A A C A G T T G C A T T A G G T G A T CT A T
A G G T A A G G G C T T T T T C T C C T C C C C A T A C C C T T A A G A A T A G C T C T G T G T A T G T G A A A G A G T G G G T G A T G T T
T T T G G C T A A A T A C T T T T T T C A T T T T A C C A A G A A A C T G A A A T A A C T T T G G C C A G G A G A A A A T A C T T C C T G A
A C A T T T T A T T T C T C T T T T T C A A G A A A A A G A C A G A G G A T T T T C C T T G A G T C C C T G C T A A A A C A T T C C A T T G
T T A A A A T T T G C A C T T T G A A G G T A A G C T T T T T A G G C C T G A C C C T C C A G G T G T C A A T G G A C T T G T G C T A C T A
T A T T T T T T A A T C T T G G T A T C A G T T A A A A G T T C A G A C A A G G C C C A C A G A A A A A G A T G T T C C A A G C A T T T G C
A A A T C T C A G C C G G T T A C A C A A A C C T T C T T T T T T A C A T C C A C T T G C T T A T A T T A T T A C C A T C A C T T T A C G T
C A T T C C T C A A C T A C T A T C A C A T C C A T T T A A T G A T T T C T G T A A A C A T T T T T T A A A C A G T T A G G A T G T C A C T
T A A C A T T T T T T G A G T T A G A G T C A G G G A A G C A A G C C A T G CT C A A T A T T T A A C A A T C A C T T G T A T G T A T A T G
T T T G T G G A A G AG T G T G T T T T A T T T G T C A T G T A T T G G T A C A A G C A GA T G C A G T A T A A A C T C A C A A A C A C A G
A T T T G A A A A T A A T G C A C A C A G T G T T C A A A T T T G A A C T T T T C T C A T G G A T T T C A T G G T A TG G G C CA A C A T G
G T G T T T A C A T T A T A T A A T T C C T G C T G T G C A A G T A A A G C A C A C T T T T T T T T C C C T A A A A T T T T T T T T C C C C
A T G T A T C C T A T T A T G G A T A C T A G T T T T G T T A A T T A T G A T T C T T T T T TT T C T C T T T T C T T T T T T T T T A G A A
T A T A G C A G T T A A T G C T A T T G C T G A A A T G A A T G A T T T T T T T T T C T G A A A T A T A A T C A T T G A T G C T T G A A T G
A T A G A A T T T T A GT A C T G T A A A C A G G C T T T A G T A A T T A A T G T G A G A G A C T T A G A A G A A A T G C T T A G A A T G G
A C T A T T G A G T G T G C C T A A A T G A A T T C T G C A G T A A C T G G T A T T C T T G G T T T T G T G C T A C T T A A T A C A C A T T
A A T T C A G A A A C T G T A T T C T G T T A T G A G T T T G A C A G T C T T T T G G A G C A A C C A A C A A C T T T G A T G T T T GC A C
T A A G A T T T T A T T T G G A A TG C A A G A G A G G T T G A A A GA G C T T T C A G T A G T A C A C A T G T A A C T A A T T T A T T T G
A A A T C T G T C C T A A A G A A A T C T A C C A G T T T G T T C A A A T G C C T T T T T A A A A T T C A T C A C A G A T A A T T A G T G A
A A A C G C A G A G T A T C G T A C T T T T C T T C T T G CA T G T C A A CA A C A T A A A CA G T T T T G T A C A A T G A G A A A T A C T
A A T T T G T T T G A C A T T C C A T A T T A A A C T A C T G T C A T G T T C A G C T T C A T T G C A T G T A A T G T A G A C T A G T C C A
T C A G A T C A T G T G T T T C T G G A G A G T G T T C T T T A T T C A A T A A A G T T T T A A T T T A G T A T A A A G A G A G C C T C T A
A A A A A A A A A A A A A A A A A
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