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Difference between DNA of Prokaryotes and Eukaryotes


Prokaryotes and eukaryotes are among the broad species in an ecosystem. Almost all organisms are classified into either prokaryotes or eukaryotes. You’ll see entirely different DNA structures and compositions in both classes that we will discuss today. But, first, comprehend what a DNA structure is and why it is necessary for survival.

An organic double-stranded chemical compound that encodes genetic information for the development, functioning, and reproduction of organisms is called Deoxyribonucleic Acid or DNA. All organisms contain DNA in their cells. It plays an essential role in passing heredity characteristics from parents to offspring. The primary difference between the DNA of prokaryotes and eukaryotes is that the former has a small amount of it while the latter acquires an ample amount. Let’s check out other differences through a comparison table.


Comparison Table

ParametersProkaryotic DNAEukaryotic DNA
PresenceIn CytoplasmIn Nucleus
DNA ShapeCircularLinear
Plasmid DNAPresentAbsent
Genome CopiesOnly OneMultiple
No. of GenesVery FewMany
No. of ChromosomesSingleMultiple
Histone PackagingAbsentPresent
Replication SiteInside CytoplasmInside Nucleus
Nature of ReplicationContinuous ProcessOccurs During the S Phase of Cell Cycle
Efficiency of ReplicationRapidSlow
Presence of TransposonsPresentAbsent
DNA SizeEqual to or Less than 0.1pgMore than 0.1pg
Repetitive SequencesLessMore/Common
mRNA’s NaturePoly-cistronic mRNAMono-cistronic mRNA

Define Prokaryotic DNA

DNA of prokaryotic cells is observed as circular, under a microscope, which is diffused in the dense cytoplasmic region called the nucleoid. These structures are always present freely floating in the cytoplasm. In addition to chromosomal DNA, small, single-stranded circular plasmid DNA also contains additional information and certain genes like antibiotic resistance genes.

Such genes are essential for bacterial survival. Plasmid DNA is an essential component in genetic engineering techniques. The major region of the prokaryotic genome retains protein information due to its small size. Unlike eukaryotic DNA, prokaryotic DNA is not supercoiled because they lack histone protein structures.

Depending upon the species, the DNA of prokaryotes ranges from a few thousand to a million base pairs (about 160,000-12.2000000 bp). Prokaryotic DNA is enriched with a group of genes, called operons, presenting a common promoter and terminator sequence. In other words, prokaryotic DNA is without introns (non-coding sequences) or non-functional DNA. These circular DNA structures have only one origin of replication, unlike eukaryotes. Hence, the process forms single replication folk and bubbles. Furthermore, the replication process in prokaryotes is more efficient, having a speed of 2000 nucleotides/second. Many active transposons are present in prokaryotes, which are moveable genetic components and involved in genetic diversity.

Define Eukaryotic DNA

The DNA of eukaryotes is properly enclosed in the nuclear/plasma membrane, found in the nucleus. Some membrane-bound organelles like mitochondria and chloroplast also have quantities of DNA; however, these DNAs are not the genomic part. The eukaryotic DNA stays linearly in the chromatin structure when the cell is not dividing. Soon as the organism starts its meiosis stages, the chromatin material condenses and forms a rod-shaped body called a chromosome. These bundles of chromosomes have DNA packed inside them. Furthermore, the DNA of eukaryotes is attached to complex histone proteins that eventually give them a compound structure. Eukaryotic DNA contains telomeres at both ends, which are repetitive sequences of nucleotides and protect chromosomes from deterioration.


A large number of base pairs is present in the chromosomes of eukaryotes. Eukaryotes are multicellular organisms, so they contain several copies of the genome. The amount of non-functional DNA in eukaryotic cells is high due to low gene density, which plays an essential role in gene expression. Unlike prokaryotes, eukaryotic genes have non-coding sequences known as introns. There are multiple origins of replications present with low efficiency (100 nucleotides/second). Contrary to prokaryotic DNA, the mobile genetic elements, i.e., transposons, are absent in the eukaryotic genome.

Similarities between the DNA of Prokaryotes and Eukaryotes

Eukaryotic organisms are multicellular and more complex in traits than unicellular prokaryotic organisms. A few similarities exist between prokaryotic and eukaryotic DNA:

  • Both carry out the processes of replication, transcription, and translation.
  • The ribosome is the site of translation in both organisms.
  •  mRNA, a type of RNA, acts as the primary unit of transcription in both.
  • For base-pairing, the DNA of both domains follows the rule of Chargaff.
  • Extra-chromosomal genetic material is present in both. Prokaryotes have plasmid DNA, whereas it’s mitochondrial DNA in eukaryotes.

Due to the complexity and similarity in their traits, both domains of organisms differ a lot based on the nature of DNA present in them. Following are the differences between the DNA of eukaryotic and prokaryotic organisms.


Differences between DNA of Prokaryotes and Eukaryotes


Prokaryotic DNA

DNA of prokaryotes is found inside the cell’s cytoplasm, in the dense nucleoid region.

Eukaryotic DNA

On the contrary, the DNA of eukaryotes is found enclosed in the nucleus, covered by the nuclear/plasma membrane, of the cell.

Size of DNA

Prokaryotic DNA

The size of prokaryotic DNA is about or less than 0.1 pg.

Eukaryotic DNA

Contrary to prokaryotes, eukaryotes have DNA of size more than 0.1 pg.

Presence in Other Organelles

Prokaryotic DNA

The DNA of prokaryotic cells is absent in other organelles of a cell.

Eukaryotic DNA

On the other hand, eukaryotic DNA is found in other cell organelles, e.g., mitochondria.

Form/Shape of DNA

Prokaryotic DNA

The genome of prokaryotic cells is present in a circular form.

Eukaryotic DNA

Similarly, the genome of a eukaryotic cell is present in linear form with two ends.

Number of Genes

Prokaryotic DNA

Prokaryotic DNA contains a few genes with no introns present.

Eukaryotic DNA

In contrast, eukaryotic DNA has many genes where introns (non-coding sequences) are also present.

No. of Chromosomes & Genome Copies

Prokaryotic DNA

These DNAs are organized into a single chromosome, having only one copy of the genome.

Eukaryotic DNA

Unlike the above, these DNAs are organized into several chromosomes with more than one genome copy.

Non-Functional DNA

Prokaryotic DNA

As the prokaryotic DNA is not packed with histone proteins, it has less non-functional DNA.

Eukaryotic DNA

Likewise, eukaryotic DNA is properly packed with complex histone structures, so the amount of non-functional DNA is high.

Site & Origin of Replication

Prokaryotic DNA

The replication of prokaryotes occurs inside the cytoplasm with a single origin.

Eukaryotic DNA

On the contrary, the replication of eukaryotes occurs inside the nucleus with multiple origins.

The Efficiency of Replication Process

Prokaryotic DNA

DNA Replication process is less complex due to the small size of the prokaryotic genome; hence replication rate is rapid.

Eukaryotic DNA

In contrast, the replication process of eukaryotic DNA is more complex due to its larger genome size. Hence, the replication rate is slow.

Amount of GC/AT

Prokaryotic DNA

More GC content as compared to AT is present in prokaryotic DNA.

Eukaryotic DNA

Whereas eukaryotic DNA has more AT content than the GC.

ConclusionThe different types of cells in nature have DNA present in them, without which life is impossible. DNA of prokaryotes and eukaryotes differ based on quantity, packing, replication, and information content. Prokaryotic DNA is organized in a single circular chromosome, while in eukaryotes, DNA is arranged in several linear chromosomes. The replication process is similar to the DNA of prokaryotes and eukaryotes, but this process is comparatively simpler in prokaryotes. The eukaryotic genome is larger and more complex than the prokaryotic genome.

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